Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rafael Montenegro Mar??n
Date created: 2016-10-16 11:00:00
Date modified: 2016-10-19 10:00:51
Golden Gate Adapter
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K1903018_sequence (Version 1) |
Description
This parts is composed of a random sequence of 100 base pairs follow upstream and downstream by a BsaI restriction site. The fusion sites for both ends are part of the standard prefix and the suffix. The part has the advantage to be clone into a RFP plasmid with RFC 10 and then used as the vector for the Golden Gate reaction.Notes
We had a random sequence into the BsaI cut sites to make a sequence easy to detect after a PCR reaction and leading the enzyme digestion. For further design a good option is to change the random sequence with a RFP o GFP device, using J23101 for example, to have a selection in the transformation.Source
The restriction site sequence was obtained from NEB enzyme finder| Sequence Annotation | Location | Component / Role(s) |
| Golden Gate Adapter | 1,113 | feature/misc sequence_feature |