BBa_K1903019

BBa_K1903019 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1903019

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Rafael Montenegro Mar??n

Date created: 2016-10-16 11:00:00

Date modified: 2016-10-17 08:06:56

Intein N SSp DnaB S1

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1903019_sequence (Version 1)

Description

This part is composed by the 11 amino acids from the SSp DnaB Intein N and 5 amino acids from the original Extein

Notes

Reverse translate to the organism of interest.

Needs an ATG codon to start transcription if wanted to express itself apart.

Source

The sequence of amino acids: LRESGCISGDSLISLA was reverse translate to E. coli

The amino acid sequence was obtained from:
Volkmann, G., Volkmann, V., & Liu, X. Q. (2012). Site‐specific protein cleavage in vivo by an intein‐derived protease. FEBS letters, 586(1), 79-84.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Int N DnaB	1,48	sequence_feature, feature/misc

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1903019/1

Member of these Collections

• iGEM Parts Registry