BBa_K1904002

BBa_K1904002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1904002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Enrique Chimal Juarez, Roger Milton Rubio S??nchez, Enrique Palomino, Brian Morteo

Date created: 2016-06-06 11:00:00

Date modified: 2016-06-07 09:29:21

Mutated endolysin specific for Acinetobacter baumannii with terminator. C-terminal HisTag

Details

• Types: DnaRegion
• Roles: Intermediate; engineered_region
• Sequences: BBa_K1904002_sequence (Version 1)

Description

This biobrick is composed by the mutated endolysin (BBa_K1904000) assembled with a double terminator (B0015), BBa_K1904002 is in RFC 25 standard to allow protein engineering. As the part does not contain a promoter it should be cloned into a promoter-containing plasmid for its usage. Additionally the part was complemented with a C-terminal HisTag in order to allow its purification via a Nickel-affinity chromatography.

Notes

This sequence was optimised for its usage in RFC 25 standard and in E. coli as chassis.

Source

Acinetobacter baumannii phage Abp1.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
K1904000	1,552	feature/cds, CDS
His Tag	553,570	tag, feature/tag
B0015	571,702	feature/BioBrick, engineered_region

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1904002/1

Member of these Collections

• iGEM Parts Registry