| Types | DnaRegion
|
| Roles | engineered_region
Intermediate
|
| Sequences | BBa_K1904004_sequence (Version 1)
|
Description
This biobrick contains the coding sequence for a T4 Holin (BBa_K112805) which form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part is assembled with B0034 (RBS). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the holin is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part.
Notes
This biobrick was optimised for its usage in RFC 25 standard and in E. coli, as chasis. It is designed as an N-part and it does not contain restriction site for NgoMIV.
Source
T4 bacteriophage
