Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Enrique Chimal Juarez, Roger Milton Rubio S??nchez, Enrique Palomino, Brian Morteo
Date created: 2016-06-06 11:00:00
Date modified: 2016-06-07 06:26:20
Fused protein (Holin/Endolysin) with RBS and terminator, C-terminal HisTag, (RFC 25 N-Part).
| Types | DnaRegion |
| Roles | engineered_region Intermediate |
| Sequences | BBa_K1904005_sequence (Version 1) |
Description
This part contains the coding sequences of BBa_K1904002 and BBa_K1904004 which consist of an specific endolysin for Acinetobacter baumannii and a T4 holin, forming a fusion protein. Additionally the part is assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the biobrick is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part. Ultimately a C-terminal HisTag was added in order to allow its purification via a Nickel-affinity chromatography.Notes
This sequence was optimised for its usage in RFC 25 standard and in E. coli as chassis. It is designed as a Freiburg N-part and it does not contain restriction site for NgoMIV.Source
Acinetobacter baumannii phage Abp1 and bacteriophage T4| Sequence Annotation | Location | Component / Role(s) |
| B0034 scar K1904004 scar K1904002 His Tag stop B0015 | 1,12 13,18 19,672 673,678 679,1230 1230,1248 1249,1251 1252,1380 | feature/BioBrick engineered_region feature/scar feature/BioBrick engineered_region feature/scar engineered_region feature/BioBrick tag feature/tag stop_codon feature/stop feature/BioBrick engineered_region |