Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Juan Carlos Rueda Silva
Date created: 2016-09-12 11:00:00
Date modified: 2016-09-13 10:11:39
Riboswitch to detect E5 mRNA from HPV-16 and express RFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1905003_sequence (Version 1) |
Description
DNA coding for a riboswitch to detect E5 mRNA from Human Papillomavirus type 16 and express Red Fluorescent Protein. This part includes a promoter (BB_R0010) and a RBS (BBa_B0034), the reverse complementary for a section of E5 DNA coding region, and the original sequence with one base changed, RFP coding region (BBa_E1010) and a terminator (BBa_B0015). When there is presence of E5 mRNA, the riboswitch turns on and it allows the expression of RFP as reporter protein.Notes
The main design consideration is the proper fold of the mRNA and hybridization with E5 mRNA.Source
It does not come from a genomic sequence, it assembled and engineered in order to fold and unfold in presence or absence of E5 mRNA and code RFP.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1905000 mrfp1 Help:Barcodes BBa_E1010 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 | 1,277 284,958 965,989 284,989 998,1077 1009,1052 1086,1126 1093,1112 1113,1126 1119,1119 998,1126 | engineered_region feature/BioBrick feature/cds CDS feature/barcode engineered_tag engineered_region feature/BioBrick feature/BioBrick engineered_region stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon feature/BioBrick engineered_region |