Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Juan Carlos Rueda Silva
Date created: 2016-09-12 11:00:00
Date modified: 2016-09-13 09:55:42
Riboswitch to detect L1 mRNA from HPV and express BFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1905004_sequence (Version 1) |
Description
DNA coding for a riboswitch to detect L1 mRNA from Human Papillomavirus and express Blue Fluorescent Protein. This part includes a promoter (BB_R0010) and a RBS (BBa_B0034), the reverse complementary for a section of L1 DNA coding region, and the original sequence with one base changed, BFP coding region (BBa_K592009) and a terminator (BBa_B0015). When there is presence of L1 mRNA, the riboswitch turns on and it allows the expression of BFP as reporter protein.Notes
The main design consideration is the proper fold of the mRNA and hybridization with L1 mRNA.Source
It does not come from a genomic sequence, it assembled and engineered in order to fold and unfold in presence or absence of L1 mRNA and code BFP.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1905001 amilCP BBa_K592009 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 | 1,283 290,955 290,958 967,1046 978,1021 1055,1095 1062,1081 1082,1095 1088,1088 967,1095 | feature/BioBrick engineered_region feature/cds CDS engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon feature/BioBrick engineered_region |