| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K1905005_sequence (Version 1)
|
Description
DNA coding for a riboswitch to detect E6 mRNA from Human Papillomavirus type 16 and type 18 and express Yellow Fluorescent Protein. This part includes a promoter (BB_R0010) and a RBS (BBa_B0034), the reverse complementary for a section of E6 DNA coding region, and the original sequence with one base changed, YFP coding region (BBa_K592010) and a terminator (BBa_B0015). When there is presence of E6 mRNA, the riboswitch turns on and it allows the expression of YFP as reporter protein.
Notes
The main design consideration is the proper fold of the mRNA and hybridization with E6 mRNA
Source
It does not come from a genomic sequence, it assembled and engineered in order to fold and unfold in presence or absence of E6 mRNA and code YFP.
