BBa_K1911003

BBa_K1911003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1911003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jack Kwon
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-15 11:01:10

ClpX from E. Coli



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1911003_sequence (Version 1)

Description

ClpX is part of the protein-degradation system called ClpXP. It works in unison with another protein called ClpXP so that it can de-linearize and unfold proteins into amino acids which can then be recycled by the cells. In E. coli cells, ClpXP focuses on degrading incorrect proteins and reducing them down into amino acids which can be recycled by the cells. ClpXP recognizes the incorrect proteins by certain SsrA-tags which are attached at the ends of incorrect proteins. ClpX in unison with ClpP can be used to degrade unwanted proteins and can be used in future projects in conjunction with certain degradation tags.

Notes

There were illegal restriction sites which cut the ClpX gene into smaller sequences, rendering it obsolete. So we had to introduce wobbles to eliminate these illegal restriction sites so that ClpX may be expressed and allowed to function as a complete protein.

Source

ClpX is found naturally in the E. coli genome.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1911003/1