BBa_K1913001

BBa_K1913001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1913001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lisa R??ttjers
Date created: 2016-09-21 11:00:00
Date modified: 2016-09-22 06:27:24

chiB for Varroa destructor



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1913001_sequence (Version 1)

Description

Serratia marcescens GEI chiB protein coding region optimized for BioBrick use. This part is useful for breaking down chitin polymers. The strain of origin has been isolated from the Chinese honey bee and showed virulence towards Varroa destructor. Chitinases A and B from the strain were found to have miticidal activity. ChiB has exochitinase activity. This protein is known to show synergistic activity with ChiA. ChiB can potentially be used to control Varroa destructor mite populations, as mite mortality increases when the mites are subjected to filter paper incubated with purified chiB.

Notes

This sequence was optimized using the IDT codon optimization tool. One restriction site was removed; PstI at position 1049. Additionally, several antisense stop codons were introduced to prevent antisense transcription.

Source

This part is based on chiB from Serratia marcescens GEI; accession GQ855218.

Sequence Annotation Location Component / Role(s)
ATG start codon
TAA stop codon
TAA stop codon
chiB coding region
1,3
1498,1500
1501,1503
1,1503
feature/start start_codon
feature/stop stop_codon
stop_codon feature/stop
feature/cds CDS
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1913001/1