Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianhe Wang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 03:45:19
Synthetic plac-FixK2 hybrid promoter with RBS
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1913023_sequence (Version 1) |
Description
Synthetic plac-FixK2 hybrid promoter is the promoter that could not only be activated by light sensors YF1-FixJ composite and but inhibited by LacI repressor. The sequence of upper control element is from wild type FixK2 hybrid promoter on genomic sequence of Bradyrhizobium japonicum, which contains two typical FixJ boxes. And core element region is replaced by core element region of ompC promoter from another two-component systems EnvZ-OmpR in E. coli. Addition lac operators on the both side of promoter as second control element that results in transcription repression.Notes
However, according to some previous iGEM projects (UNITN-Trento 2013, INSA-Toulouse 2013), the transcription activity of the wild type Fixk2 promoter is so weak that they all added an inverter part to control their target gene expression. Even the original pDusk system in darkness has only 5 times expression levels than in light conditions. Therefore, we decided to enhance the transcriptional activity of the Fixk2 promoter by changing the core element region of the wild type Fixk2 by switching core element region into ompC promoter -10 to -35 that from another two component system, because we couldn???t guarantee that FixJ boxes could be compatible with the core element of a constitutive promoter.Source
Genomic sequence of Bradyrhizobium japonicum.| Sequence Annotation | Location | Component / Role(s) |
| conserved BBa_B0034 FixJ box FixJ box lac operator ompC core element lac operator | 5,8 151,162 75,88 41,48 1,21 89,129 130,150 | feature/conserved conserved_region engineered_region feature/BioBrick non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding operator feature/operator feature/misc sequence_feature operator feature/operator |