Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianhe Wang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 03:46:38
Synthetic ptet-FixK2 hybrid promoter with RBS
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1913024_sequence (Version 1) |
Description
Synthetic ptet-FixK2 hybrid promoter is the promoter that could not only be activated by light sensors YF1-FixJ composite and but inhibited by TetR repressor. The sequence of upper control element is from wild type FixK2 hybrid promoter on genomic sequence of Bradyrhizobium japonicum, which contains two typical FixJ boxes. And core element region is replaced by core element region of BBa_J23106. Addition tet operators are inserted into core element region of promoter as second control element that results in transcription repression.Notes
According to some previous iGEM projects (UNITN-Trento 2013, INSA-Toulouse 2013), the transcription activity of the wild type Fixk2 promoter is so weak that they all added an inverter part to control their target gene expression. Even the original pDusk system in darkness has only 5 times expression levels than in light conditions. Therefore, we decided to enhance the transcriptional activity of the Fixk2 promoter by changing the core element region of the wild type Fixk2 by adding a strong constitutive promoter (BBa_J23106) from iGEM part registry and by adding two typical FixJ boxes into the -40 to -70 region.Source
genomic sequence of Bradyrhizobium japonicum| Sequence Annotation | Location | Component / Role(s) |
| conserved BBa_B0034 FixJ box FixJ box tet operator tet operator -10 -35 | 5,8 124,135 20,27 54,67 74,92 99,117 93,98 68,73 | feature/conserved conserved_region feature/BioBrick engineered_region non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/operator operator feature/operator operator non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site |