Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianhe Wang
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-14 03:31:57
Wild type plac-FixK2 hybrid promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1913025_sequence (Version 1) |
Description
Wild type plac-FixK2 hybrid promoter is the promoter that could not only be activated by light sensorsYF1-FixJ composite and but inhibited by LacI repressor. The sequence of upper control element and core element region are from wild type FixK2 hybrid promoter on genomic sequence of Bradyrhizobium japonicum. Addition lac operators on the both side of promoter as second control element that results in transcription repression.Notes
In order to make Fixk2 promoter as an inducible promoter for the toggle switch,we had to add additional repressor operator sequences into the Fixk2 sequence, they are both on upstream and downstream the Fixk2 sequence, which could be bonded by tetrameric Lac repressor resulting in the formation of a DNA loop and consequently on transcription repression.Source
Genomic sequence of Bradyrhizobium japonicum| Sequence Annotation | Location | Component / Role(s) |
| Lac operator FixJ box FixJ box Lac operator -10 -35 RBS | 1,21 64,67 90,97 132,152 120,120 98,98 153,157 | operator feature/operator non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding operator feature/operator feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site ribosome_entry_site feature/rbs |