Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianhe Wang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 03:29:38
Wild type ptet-FixK2 hybrid promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1913026_sequence (Version 1) |
Description
Wild type ptet-FixK2 hybrid promoter is the promoter that could not only be activated by light sensorsYF1-FixJ composite and but inhibited by TetR repressor. The sequence of upper control element and core element region are from wild type FixK2 hybrid promoter on genomic sequence of Bradyrhizobium japonicum. Additional tet operators are inserted in the core element region of promoter as second control element that results in transcription repression.Notes
In order to make Fixk2 promoter as an inducible promoter for the toggle switch, we had to add additional repressor operator sequences into the Fixk2 sequence, they are both inserted in the core element region of the Fixk2 sequence, which could be bonded by dimerized TetR repressors resulting in the formation of steric hindrance and consequently on transcription repression.Source
Genomic sequence of Bradyrhizobium japonicum| Sequence Annotation | Location | Component / Role(s) |
| FixJ box FixJ box tet operator tet operator RBS | 43,46 69,76 82,100 107,125 133,137 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/operator operator feature/operator operator ribosome_entry_site feature/rbs |