Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 10:33:56
NpF2164g5 + intein-CBD tag
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1916000_sequence (Version 1) |
Description
A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain attached to the C-terminus for purification. As with all CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and appears vibrant blue-green under normal light.Notes
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.Source
From cyanobacteria genome| Sequence Annotation | Location | Component / Role(s) |
| NpF2164g5 intein/chitin binding domain | 1,513 514,1296 | feature/protein CDS feature/protein CDS |