BBa_K1916000

BBa_K1916000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1916000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jonathan Chen

Date created: 2016-10-08 11:00:00

Date modified: 2016-10-09 10:33:56

NpF2164g5 + intein-CBD tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1916000_sequence (Version 1)

Description

A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain attached to the C-terminus for purification. As with all CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and appears vibrant blue-green under normal light.

Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.

Source

From cyanobacteria genome

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
NpF2164g5	1,513	feature/protein, CDS
intein/chitin binding domain	514,1296	feature/protein, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1916000/1

Member of these Collections

• iGEM Parts Registry