BBa_K1916109

BBa_K1916109 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1916109
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 11:22:44

Ara-Inducible Osc7112_5903g3 Expression System



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1916109_sequence (Version 1)

Description

The Osc7112_5903g3 protein (see BBa_K1916009) under control of a strong pBAD promoter. RFC10 compatible.

Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.

Source

Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.

Sequence Annotation Location Component / Role(s)
BBa_K206000
conserved
BBa_B0034
osc7112_5903g3
intein/chitin binding domain
BBa_K1916009
1,130
143,146
139,150
157,798
799,1581
157,1581
feature/BioBrick engineered_region
feature/conserved conserved_region
engineered_region feature/BioBrick
CDS feature/protein
feature/protein CDS
feature/BioBrick engineered_region
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1916109/1