Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 01:03:09
Ara-Inducible CBCR3 Expression System
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1916113_sequence (Version 1) |
Description
The CBCR3 protein (see BBa_K1916013) under control of a strong pBAD promoter. RFC10 compatible.Notes
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.Source
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.| Sequence Annotation | Location | Component / Role(s) |
| promoter AraI1 AraI2 BBa_K206000 conserved BBa_B0034 CBCR3 intein/chitin binding domain BBa_K1916013 | 1,131 40,57 61,78 1,130 143,146 139,150 157,678 679,1461 157,1461 | feature/promoter promoter feature/operator operator feature/operator operator engineered_region feature/BioBrick conserved_region feature/conserved engineered_region feature/BioBrick feature/protein CDS feature/protein CDS feature/BioBrick engineered_region |