BBa_K1916119

BBa_K1916119 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1916119
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Chen
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 12:54:40

Ara-Inducible CBCR8 Expression System



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1916119_sequence (Version 1)

Description

The CBCR8 protein (see BBa_K1916019) under control of a strong pBAD promoter. RFC10 compatible.

Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.

Source

Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.

Sequence Annotation Location Component / Role(s)
promoter
AraI1
AraI2
BBa_K206000
conserved
BBa_B0034
CBCR8
intein/chitin binding domain
BBa_K1916019
1,131
40,57
61,78
1,130
143,146
139,150
157,726
727,1509
157,1509
promoter feature/promoter
feature/operator operator
operator feature/operator
feature/BioBrick engineered_region
feature/conserved conserved_region
feature/BioBrick engineered_region
CDS feature/protein
feature/protein CDS
engineered_region feature/BioBrick
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1916119/1