Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shiji Zhao
Date created: 2016-09-06 11:00:00
Date modified: 2016-10-14 10:12:45
T7 promotor (under the control of lacI) and downstream CecropinXJ
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1919003_sequence (Version 1) |
Description
Antimicrobial peptide CecropinXJ belongs to AMP family Cecropin, a group of small basic polypeptides mainly found in the hemolymph of insects, consist of 31-39 amino acid residues and have a broad spectrum, high heat stability and potent bacteriostatic activity.Notes
The coding sequence was optimized for E.coli expression using JCat http://www.jcat.de/. The single stop codon was replaced by double TAA stop codon.Source
The original sequence of CecropinXJ comes from GenBank NM_001043995.1. The sample used for experiments was artificially synthesized by Tsingke.| Sequence Annotation | Location | Component / Role(s) |
| lacI promotor T7 promotor lac operator TrxA 6xHis Tag Thrombin site S tag enterokinase site cecropinXJ T7 terminator rrnB T1 terminator lacI | 1084,1161 1474,1492 1493,1517 1562,1888 1910,1927 1937,1954 1961,2005 2021,2035 2057,2245 2342,2389 2399,2444 1,1083 | promoter feature/promoter promoter feature/promoter feature/operator operator feature/tag tag feature/tag tag feature/tag tag feature/tag tag feature/tag tag CDS feature/cds feature/stop stop_codon stop_codon feature/stop CDS feature/cds |