Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zekun Lv
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-18 09:59:58
The device consist of temperature promotor and hokD coding gene.
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1919100_sequence (Version 1) |
Description
This device is a combination of two parts submitted by previous igem teams that one （BBa_K608351） is a temperature-regulated promoter followed by the other （BBa_K1497008 ）that a gene coding for a protein hokD which induces cell lysis. The promoter we chose won???t start translation until the temperature is raised to 37℃. And at 42℃ can we get the peck efficiency of expression. The promoter is in fact a composite sequence that preceding the regulated promoter is the control sequence which can synthesis λ cI repressor. λ cI repressor binds with the promoter sequence when environment temperature is 28~30℃ while at 42℃, the protein denatures and falls off by which initiate the transcription. We use the temperature-regulated promoter to express hokD gene. The hokD gene of??E. coli??transcribes for a small polypeptide that causes cell death by elimination of vital cell wall functions.In short , our device can induce cell lysis when temperature is raised to 42℃.
To deliver the antimicrobial peptides in the right place and at the right time, we designed and constructed this temperature-regulated device. When put under the sole of feet, our peptide-expressing E.coli cells in the insole would be incubated at a warmer environment due to body temperature. We use this promoter to control the synthesis of hokD for the raised temperature will promote the translation of hokD which following the promoter.
Notes
We use pSB1C3 vector to express this device for its more avaliable.Source
we acquired that two parts BBa_K608351 and BBa_K1497008 we based on on igem distribution plates and constructed the composite device through molecular biology technique.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K608351 BBa_K1497008 | 1,948 956,1112 | feature/BioBrick engineered_region feature/BioBrick engineered_region |