| Types | DnaRegion
|
| Roles | Device
engineered_region
|
| Sequences | BBa_K1919301_sequence (Version 1)
|
Description
LivF encodes a subunit of leucine ABC tansporter. This device constructed in our team contains a promoter J23100, a RBS, livF coding region and a terminator. The device can be transferred into E.coli and overexpress livF directly. The expression of the livF RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been overexpressed in E.coli BL21 and E.coli DH5a.
Notes
The restriction sites are eliminated by mutation.
Source
The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company.
