| Types | DnaRegion
|
| Roles | Device
engineered_region
|
| Sequences | BBa_K1919302_sequence (Version 1)
|
Description
LivG is an ATP-binding cassette (ABC) protein that is a component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. The device encodes livG protein, one of a subunit of leucine ABC transporter. The LivG protein sequence contains Walker A and Walker B motifs involved in the binding and hydrolysis of ATP.
Notes
The restriction sites are eliminated by mutation.
Source
The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company.
