BBa_K1919303

BBa_K1919303 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1919303

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shaobo Yang

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-21 12:11:10

livH

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1919303_sequence (Version 1)

Description

LivH is an integral membrane component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. LivH encodes a very hydrophobic protein with 7 or 9 predicted transmembrane regions.
This device constructed in our team contains a promoter J23100, a RBS, livH coding region and a terminator. The device can be transferred into E.coli and overexpress livH directly. The expression of the livH RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livH has been highly expressed in E.coli BL21 and E.coli DH5a.

Notes

The restriction sites are eliminated by mutation.

Source

The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
J23100	1,35	engineered_region, feature/BioBrick
B0030	44,58	engineered_region, feature/BioBrick
livH coding region	65,992	engineered_region, feature/BioBrick
B0010	993,1071	feature/BioBrick, engineered_region

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1919303/1

Member of these Collections

• iGEM Parts Registry