Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shaobo Yang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-21 12:12:25
livK
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1919304_sequence (Version 1) |
Description
LivK is the periplasmic binding protein of the LS or leucine specific ABC transport system in E. coli K-12.LivK is synthesized as a precursor; a 23 amino acid signal sequence is cleaved upon export to the periplasm.The protein consists of two domains connected by a 3-stranded hinge; in the closed form, ligands (phenylalanine and leucine) are bound in a cleft formed between the two domains; binding is stabilized by hydrogen bonds and van der Waals contacts.This device constructed in our team contains a promoter J23100, a RBS, livK coding region and a terminator. The device can be transferred into E.coli and overexpress livK directly. The expression of the livK RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livK has been highly expressed in E.coli BL21 and E.coli DH5a.