BBa_K1919305

BBa_K1919305 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1919305

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shaobo Yang

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-21 12:14:01

livM

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1919305_sequence (Version 1)

Description

LivM is an integral membrane component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. livM encodes a very hydrophobic protein with 9 predicted transmembrane regions.
This device constructed in our team contains a promoter J23100, a RBS, livM coding region and a terminator. The device can be transferred into E.coli and overexpress livM directly. The expression of the livM RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livM has been highly expressed in E.coli BL21 and E.coli DH5a.

Notes

The restriction sites are eliminated by mutation.

Source

The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
J23100	1,35	feature/BioBrick, engineered_region
B0030	44,58	engineered_region, feature/BioBrick
livM coding region	65,1343	engineered_region, feature/BioBrick
B0010	1344,1422	feature/BioBrick, engineered_region

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1919305/1

Member of these Collections

• iGEM Parts Registry