Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hongbin Yu
Date created: 2016-09-11 11:00:00
Date modified: 2016-10-14 07:59:48
K1321200+J04450 (Vitreoscilla hemoglobin + mRFP)
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K1919500_sequence (Version 1) |
Description
To measure the characters of Vitreoscilla haemoglobin generator constructed by iGEM14_Imperial (BBa_K1321200). Vitreoscilla haemoglobin can promote the cell growth and protein production ability. We ligate mRFP generator with VHb generator to test the protein production ability in E. coli.Notes
The Vitreoscilla haemoglobin gene sequence in iGEM database is not as the same as that in Genebank but it works.Source
iGEM HQ.| Sequence Annotation | Location | Component / Role(s) |
| J23101 constitutive BBa_K1321200 B0034 Start VHb Stop BBa_R0010 BBa_J04450 CAP lac promoter BBa_B0034 mrfp1 BBa_E1010 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop lac operator | 1,35 1,504 44,55 64,66 64,504 502,504 513,712 513,1581 601,638 649,679 721,732 739,1413 739,1419 1453,1532 1462,1507 1541,1581 1548,1567 1568,1581 1575,1575 687,703 | promoter feature/promoter engineered_region feature/BioBrick feature/rbs ribosome_entry_site feature/start start_codon CDS feature/cds feature/stop stop_codon engineered_region feature/BioBrick engineered_region feature/BioBrick non_covalent_binding_site feature/binding promoter feature/promoter engineered_region feature/BioBrick CDS feature/cds feature/BioBrick engineered_region feature/BioBrick engineered_region stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop feature/binding non_covalent_binding_site |