BBa_K1920007

BBa_K1920007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1920007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chia-En Wong ,Ying-Hsin Hung , Fang-Wei Yu , Wei-Hsuan Wang
Date created: 2016-09-22 11:00:00
Date modified: 2016-10-14 09:15:06

Pcar-RBS-amilCP-TT



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K1920007_sequence (Version 1)

Description

Glucose detection device , which uses blue chromoprotein K592009 as reportor . Pcar promoter consists of overlaping consensus CRP-binding site and consensus RNA polymerase binding site. Therefore,the steric hindrance between CRP and RNA polymerase will cause downstream gene to be repressed at high concentration of CRP. In E.coli strains,e.g.DH5α and Bl21,high concentration of CRP is present when glucose concentration is low ,resulting in increased adenylyl cyclase activity and increased cAMP . cAMP will bind the cAMP receptor protein (CRP) and CRP, in its bound form, will specifically bind the consensus CRP-binding site of Pcar promoter , resulting in downstream gene repression. In contrast , if glucose concentration is high , there will be decreased adenylyl cyclase activity ,decreased cAMP ,and decreased CRP in bound form , resulting in increased expression of downstream RFP E1010 as a reporter gene as indicator of the presence of glucose.

Notes

To edit.

Source

Biobrick

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1920007/1