Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chia-En Wong ,Ying-Hsin Hung , Fang-Wei Yu , Wei-Hsuan Wang
Date created: 2016-09-22 11:00:00
Date modified: 2016-10-14 09:15:15
Pl-RBS-amilCP-TT
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1920008_sequence (Version 1) |
Description
Glucose detection device , which uses blue chromoprotein K592009 as reportor . Pl promoter consists of overlaping consensus CRP-binding site and consensus RNA polymerase binding site. Therefore,the steric hindrance between CRP and RNA polymerase will cause downstream gene to be repressed at high concentration of CRP. In E.coli strains,e.g.DH5α and Bl21,high concentration of CRP is present when glucose concentration is low ,resulting in increased adenylyl cyclase activity and increased cAMP . cAMP will bind the cAMP receptor protein (CRP) and CRP, in its bound form, will specifically bind the consensus CRP-binding site of Pl promoter , resulting in downstream gene repression. In contrast , if glucose concentration is high , there will be decreased adenylyl cyclase activity ,decreased cAMP ,and decreased CRP in bound form , resulting in increased expression of downstream RFP E1010 as a reporter gene as indicator of the presence of glucose.Notes
To editSource
Biobrick| Access | Instance | Definition |
| public public public public | BBa_K861170 BBa_B0034 BBa_K592009 BBa_B0015 | BBa_K861170 BBa_B0034 amilCP BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K861170 BBa_B0034 BBa_K592009 BBa_B0015 | 1,36 45,56 63,731 740,868 | BBa_K861170 BBa_B0034 amilCP BBa_B0015 |