BBa_K192001

BBa_K192001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K192001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Wong, Kenny Zhan, Jason An

Date created: 2009-09-14 11:00:00

Date modified: 2015-05-08 01:11:15

CFP +tgt +lva

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K192001_sequence (Version 1)

Description

CFP with targeting sequence and LVA tag. The targeting sequence targets the protein to the encapsulin nanocompartment [1] with the aim of increasing the half-life of the protein. LVA should help limit the amount of background activity.

[1] M. Sutter, et al., "Structural basis of enzyme encapsulation into a bacterial nanocompartment," Nat. Struct. & Mol. Biol., vol. 15, pp. 939-47., 2008.

Notes

We decided to position the LVA at the C-terminus of the construct since that is its natural position. However, the tgt extension sequence is also typically found at the C-terminus, so we're hoping that the shorter LVA sequence won't interfere too much with the binding with encapsulin. If necessary, it'll be possible to remove the LVA tag via PCR.

Source

Part was synthesized.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	feature/start, start_codon
double stop	853,858	stop_codon, feature/stop
cfp	4,717	feature/protein, CDS
tgt	718,819	feature/binding, non_covalent_binding_site
lva	820,852	feature/tag, tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K192001/1

Member of these Collections

• //function/reporter/fluorescence
• iGEM Parts Registry