BBa_K1921006

BBa_K1921006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1921006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhuozhi Chen
Date created: 2016-09-30 11:00:00
Date modified: 2016-10-19 11:21:56

GCW51



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1921006_sequence (Version 1)

Description

As a cell wall protein of Pichia pastoris, GCW51 is often used as an anchor protein in Pichia pastoris surface display system because of its stable connection with the dextranomer in cell wall.?? By fusing GCW51 with the target protein expression, the target protein can be displayed on the outer of the yeast cell wall.??
By expressing the fusion protein of GCW51 protein and PETase, PETase was expressed on the surface of Pichia pastoris, and the whole cell catalyst for the degradation of PET was obtained. We made the PETase fixed on the cell wall, which can improve the stability of PETase, and is easy to control the degradation reaction of PET and PETase recycling.

Source

This part was gained from Pichia pastoris GS115.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1921006/1