U6 array

BBa_K1925004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1925004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zi Yue Wang

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-11 08:02:44

3xtetO-U6 tandom repeat(U6 array)

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1925004_sequence (Version 1)

Description

Three U6 tandom series with restricition enzyme site behind each tetO-U6,which makes it possible to drive three independent genes of interest respectively.For us,we can use it to drive three different shRNAs simutaneously.

Notes

Use proper restriction enzymes and design proper enzyme site behind and before your gene of interest

Source

Our iGEM team built it

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
tetO U6	1,338	feature/promoter, promoter
Hind III site	339,378	feature/tag, tag
tetO U6	379,716	feature/promoter, promoter
Nhe I site	717,756	tag, feature/tag
tetO U6	757,1094	promoter, feature/promoter

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1925004/1

Member of these Collections

• iGEM Parts Registry