Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zi Yue Wang
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 09:15:53
mitronic GFP backbone
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1925006_sequence (Version 1) |
Description
In order to express three shRNAs simutaneously within only one transcript,we design design artificial introns within GFP's CDS,in which we design restriction enzyme sites between each exons.Using these restriction enzyme sites we can easily insert shRNAs.Notes
Cuz its complexity of sequence，PCR will be a little difficultSource
De novo synthesis from company Genewiz| Sequence Annotation | Location | Component / Role(s) |
| GFP exon 1 intron with Age I GFP exon 2 intron with Not I GFP exon 3 intron with Sal I GFP exon 4 | 1,171 172,208 209,442 443,478 479,649 650,683 684,827 | feature/cds CDS feature/misc sequence_feature feature/cds CDS feature/misc sequence_feature feature/cds CDS sequence_feature feature/misc feature/cds CDS |