BBa_K1926011

BBa_K1926011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1926011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyi Liu

Date created: 2016-10-08 11:00:00

Date modified: 2016-10-19 01:22:24

The SNAP UNIT: SNAP-tag flanked by loxP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1926011_sequence (Version 1)

Description

This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE.


You may use this part to:

1) Localize proteins in vivo with BLOCK and dye from NEB company;

2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein;

3) Fast cut off this part by transient transfecting recombinase gene into nucleus.

Notes

None

Source

The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SNAP-tag	59,604	feature/tag, tag
loxP	1,34	non_covalent_binding_site, feature/binding
loxP	755,788	non_covalent_binding_site, feature/binding
NLS	38,58	tag, feature/tag
sv40 terminator	605,754	feature/dna, sequence_feature
TAA	626,628	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1926011/1

Member of these Collections

• //chassis/eukaryote/human
• //function/recombination/cre
• iGEM Parts Registry