BBa_K1926012

BBa_K1926012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1926012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyi Liu

Date created: 2016-10-08 11:00:00

Date modified: 2016-10-11 03:47:23

The VIKA UNIT: recombinase VIKA flanked by Vox

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1926012_sequence (Version 1)

Description

This part is the VIKA UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA. This part is the improvement of the BBa_K1641003，which is a plain VIKA.


You may use this part to:

1) Test the cut-off efficiency of recombinase VIKA by qRT-PCR;

2) Fast cut off this part by transient transfecting recombinase gene into nucleus;

3) Do lineage tracking.

Notes

None

Source

The recombinase gene VIKA and its RTS Vox were got through denovo synthesis. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdn3.1.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Vox	1,34	feature/binding, non_covalent_binding_site
VIKA	35,1117	CDS, feature/cds
Vox	1261,1294	feature/binding, non_covalent_binding_site
sv40 terminator	1118,1260	feature/dna, sequence_feature
TAA	1139,1141	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1926012/1

Member of these Collections

• //cds/enzyme/chromatinremodeling
• //chassis/eukaryote/human
• //function/recombination
• iGEM Parts Registry