PAtpI

BBa_K1930005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1930005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eike Mahlandt
Date created: 2016-10-15 11:00:00
Date modified: 2016-10-16 04:58:48

PAtpI



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1930005_sequence (Version 1)

Description

The promoter PAtpI has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 min of germination [https://www.ncbi.nlm.nih.gov/pubmed/25661487 Sinaiet al.]. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.

The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams.

Notes

The promoter PAtpI has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 min of germination [https://www.ncbi.nlm.nih.gov/pubmed/25661487 Sinaiet al.]. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.

The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams.

Source

Ordered as gBlock. IDT (Integrated DNA technologies).

Sequence Annotation Location Component / Role(s)
P(AtpI)
1,349
promoter feature/promoter
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1930005/1