BBa_K1932001

BBa_K1932001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1932001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jin Liu

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-19 09:39:39

orf1 and orf2 from Bifidobacterium

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K1932001_sequence (Version 1)

Description

Part BBa_1932001 was originally cloned from the plasmid of Bifidobacterium that contains a sequence of ori and two sequences of orf.（The plasmid had a G + C content of 62.0%, and contained two open reading frames, orf1 and orf2, likely arranged in an operon.）This sequence is essential for the shuttling of the plasmid from E.coli to Bifidobacterium.

Notes

（1）The restriction enzyme cutting site were identified.
（2）The sequences of ori and orf were confirmed.

Source

the plasmid of PMB1 from Bifidobacterium

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
orf1	1253,1570	sequence_feature, feature/misc
orf2	339,1259	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1932001/1

Member of these Collections

• iGEM Parts Registry