BclA-His

BBa_K1933000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1933000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tomoki Uchino

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-17 12:19:04

N-terminal domain of BclA protein fused to 6xHis tag

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1933000_sequence (Version 1)

Description

This part codes N-terminal domain of BclA protein which expressed on the cell surface connected by a 6??His tag to be easily identified by Western blotting.[1] Surface displayed passengers retain enzyme activity even when it is linked to BclA with 6?? His tag.

Notes

optimized to expression in E. coli K-12 strain

Source

NCBI accession number CAD56880
NCBI accession number CAD56878
NCBI accession number CAD56869
NCBI accession number CAD56870

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start codon	1,3	start_codon, feature/start
BclA	4,66	CDS, feature/cds
6xHis tag	67,84	tag, feature/tag

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1933000/1

Member of these Collections

• //cds/membrane
• iGEM Parts Registry