Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tomoki Uchino
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-17 12:46:05
INPNC fused to 6xHis tag
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1933001_sequence (Version 1) |
Description
INPNC codes for N- and C- terminal domain of Ice-Nucleation Protein from Pseudomonas syringae, connected by a 6??His tag to be easily identified by Western blotting. [1]This is used as an anchoring motif for the cell surface display of passenger domain. INPNC does not have signal peptide for anchoring on the cell surface[2] Surface displayed passengers retain enzyme activity even when it is linked to INPNC with 6?? His tag.For more information, please visit our Wiki page.
Notes
N/ASource
INPNC derived from BBa_K811005.| Sequence Annotation | Location | Component / Role(s) |
| start codon INPNC 6xHis tag stop codon | 1,3 4,957 958,975 976,978 | start_codon feature/start CDS feature/cds feature/tag tag feature/stop stop_codon |