BBa_K1935020

BBa_K1935020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1935020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Emilie Gounin

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 10:00:01

General control protein GCN4

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K1935020_sequence (Version 1)

Description

GCN4 is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'. It's naturally expressed in nuclei of Saccharomyces cerevisiae.

Tanenbaum used it in a protein tagging system for signal amplification named Sun-tag (tagging system SUperNova) where a dCas9 fused to several GCN4 appeals paratopes fused to GFP.
We also plan to use a dead Cas9 completed with several GCN4 epitopes to amplify DNA methylase activity.

Notes

in progress

Source

http://www.uniprot.org/uniprot/P03069

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GCN4 (1)	1,51	feature/protein, CDS
GCN4 (2)	73,123	feature/protein, CDS
GCN4 (3)	145,195	feature/protein, CDS
GCN4 (4)	217,267	CDS, feature/protein
GCN4 (5)	289,339	CDS, feature/protein
GCN4 (6)	361,411	CDS, feature/protein
GCN4 (7)	433,483	CDS, feature/protein
GCN4 (8)	505,555	CDS, feature/protein
GCN4 (9)	577,627	feature/protein, CDS
GCN4 (10)	649,699	CDS, feature/protein

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1935020/1

Member of these Collections

• iGEM Parts Registry