BBa_K1935020

BBa_K1935020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1935020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emilie Gounin
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 10:00:01

General control protein GCN4



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_K1935020_sequence (Version 1)

Description

GCN4 is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'. It's naturally expressed in nuclei of Saccharomyces cerevisiae.

Tanenbaum used it in a protein tagging system for signal amplification named Sun-tag (tagging system SUperNova) where a dCas9 fused to several GCN4 appeals paratopes fused to GFP.
We also plan to use a dead Cas9 completed with several GCN4 epitopes to amplify DNA methylase activity.

Notes

in progress

Source

http://www.uniprot.org/uniprot/P03069

Sequence Annotation Location Component / Role(s)
GCN4 (1)
GCN4 (2)
GCN4 (3)
GCN4 (4)
GCN4 (5)
GCN4 (6)
GCN4 (7)
GCN4 (8)
GCN4 (9)
GCN4 (10)
1,51
73,123
145,195
217,267
289,339
361,411
433,483
505,555
577,627
649,699
feature/protein CDS
feature/protein CDS
feature/protein CDS
CDS feature/protein
CDS feature/protein
CDS feature/protein
CDS feature/protein
CDS feature/protein
feature/protein CDS
CDS feature/protein
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1935020/1