Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oumnia KARIM
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-17 01:43:38
pNagP-RFP in pSBBs0K-mini plasmid
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1937006_sequence (Version 1) |
Description
(Chassis B. subtilis, carrier plasmid pSBBS0K)Length: 6843 bp
Background:
pNagP is the promoter of the NagP gene, which expresses a transporter of N-acetylglucosamine-6-phosphate in Bacillus subtilis (http://www.biocyc.org/gene?orgid=BSUB&id=BSU07700).
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
Notes
The BBa_K1937006 part was designed to demonstrate the induction of pNagP by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France).The part include the pNagP promoter and its RBS (position 840,455 to 840,656 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping. This part is a subcloning of BBa_K1937005 pNagP part in pSBBS0K_mini plasmid (BBa_K1937001).
Source
In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate (Bertram et al., 2011: https://www.ncbi.nlm.nih.gov/pubmed/21602348). This NAG molecule is the major component of chitin, a characteristic molecule of fungi.| Access | Instance | Definition |
| public public | BBa_K1937005 BBa_K1937001 | BBa_K1937005 BBa_K1937001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1937005 BBa_K1937001 | 1,1013 1022,6878 | BBa_K1937005 BBa_K1937001 |