BBa_K1937009

BBa_K1937009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1937009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oumnia KARIM
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-17 12:24:17

Epsilon/MazF : toxin and antitoxin



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1937009_sequence (Version 1)

Description

(Chassis E. coli, carrier plasmid pSB1C3)
Length: 1047 bp
Background:
This Epsilon/MazF part is one of the two components of a double toxin-antitoxin system. The part was designed to be carried on a plasmid while its counterpart MazE/Zeta was carried on another. Unfortunately, only Epsilon/MazF part was successfully cloned in E. coli.
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)

Notes

Epsilon and MazF are under the control of the promoter Pveg (constitutive promotor for B. subtilis) followed by a strong RBS. A theophylline-dependent riboswitch was used to prevent the MazE toxin expression during the cloning steps in presence of theophylline (Topp and Gallivan, 2008). Indeed, with an expressed toxin, it would be impossible to clone the Biobrick in E. coli.SacII and SalI are the restriction sites placed respectively before and after the riboswitch so that this fragment can easily be replaced depending on the control we want to apply to the bacteria.

Source

The gene Epsilon codes for a toxin countered by the anti-toxin Zeta (Zielenkiewicz and Cegłowski, 2005 ; Mutschler et al., 2011). The gene MazF encodes the anti-toxin for the MazE toxin (Bravo et al., 1987, Zhang et al., 2005, Wang et al., 2013).

Sequence Annotation Location Component / Role(s)
Pveg
Antitoxin Epsilon
RBS Antitoxin
RBS toxin
Riboswitch
Terminator
Toxin mazF
1,237
250,522
238,249
570,579
529,579
925,1004
586,924
feature/promoter promoter
feature/protein CDS
ribosome_entry_site feature/rbs
ribosome_entry_site feature/rbs
feature/misc sequence_feature
feature/stop stop_codon
CDS feature/protein
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BBa_K1937009/1