Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kyler Herrington
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-07 03:21:48
Mutant RBS with mCherry reporter gene
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1939004_sequence (Version 1) |
Description
This composite part contains an RBS with a single point mutation (C to A transversion) and the encoding region for the mCherry fluorescent reporter protein. This particular RBS mutation results in lower translational efficiency with mCherry fluorescence that is 127.83% of the native RBS.Notes
This is one of a number of random point mutation in the 12 base pair region beginning with the RBS and ending after the first nucleotide of the start codon. This was considered when designing the PCR primers.Source
This is a mutant of the RBS region of the native strain, S001, of M. maripaludis| Sequence Annotation | Location | Component / Role(s) |
| Promoter Native RBS mCherry BBa_K1383000 | 2,175 185,190 196,906 1,906 | feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/protein feature/BioBrick engineered_region |