Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K194000_sequence (Version 1)
|
Description
C-terminal domain of Saccharomyces cerevisiae cyclin 2 (CLN2) gene. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues changes the half-life of a GFP from 7h and down to 30 minutes.
Notes
The construct follow the Silverfusion standard
Source
Saccharomyces cerevisiae genomic DNA, Genbank Accession M33265