BBa_K194000

BBa_K194000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K194000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Christian Kaas
Date created: 2009-07-12 11:00:00
Date modified: 2015-05-08 01:11:16

cln2 PEST destabilization domain for rapid protein turnover



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K194000_sequence (Version 1)

Description

C-terminal domain of Saccharomyces cerevisiae cyclin 2 (CLN2) gene. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues changes the half-life of a GFP from 7h and down to 30 minutes.

Notes

The construct follow the Silverfusion standard

Source

Saccharomyces cerevisiae genomic DNA, Genbank Accession M33265

Sequence Annotation Location Component / Role(s)
Cln2 Coding sequence
1,537
feature/cds CDS
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BBa_K194000/1