BBa_K194003

BBa_K194003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K194003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Christian Kaas

Date created: 2009-10-13 11:00:00

Date modified: 2015-05-08 01:11:16

USER cassette for insertion of USER fragments

Details

• Types: DnaRegion
• Roles: sequence_feature; Other
• Sequences: BBa_K194003_sequence (Version 1)

Description

This biobrick contains a PacI/Nt.BbvCI and a AsiSI/Nb.BtsI USER cassette for insertion of PCR fragments using the USER cloning standard (see http://2009.igem.org/Team:DTU_Denmark/project). PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase.

Notes

The design follows the biobrick standard

Source

synthesized

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Nt.BbvCI	1,7	non_covalent_binding_site, feature/binding
PacI	10,17	non_covalent_binding_site, feature/binding
Nt.BbvCI	20,26	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K194003/1

Member of these Collections

• iGEM Parts Registry