BBa_K1946002

BBa_K1946002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1946002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Musa Efe Işılak

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-14 06:17:48

sgRNA targeting LacI

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_K1946002_sequence (Version 1)

Description

This part has a sgRNA targeting lacI. It also has 5' and 3' ribozyme which cleave the RNA molecule with self catalysis so that any sequence can be added to 5' or 3' to this part without preventing Cas9 binding and its activity.

Notes

.

Source

This part comes from the paper Liu, Yuchen, Yayue Zeng, Li Liu, Chengle Zhuang, Xing Fu, Weiren Huang, and Zhiming Cai. "Synthesizing AND Gate Genetic Circuits Based on CRISPR-Cas9 for Identification of Bladder Cancer Cells." Nature Communications Nat Comms 5 (2014): 5393.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
5	1,58	feature/stem_loop, stem_loop
LacI targeting region	59,78	feature/binding, non_covalent_binding_site
Cas9 recognition site	79,162	non_covalent_binding_site, feature/binding
3' ribozyme	163,205	feature/stem_loop, stem_loop

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1946002/1

Member of these Collections

• //function/crispr/cas9
• iGEM Parts Registry