| Types | DnaRegion
|
| Roles | Protein_Domain
polypeptide_domain
|
| Sequences | BBa_K1947002_sequence (Version 1)
|
Description
This sequence encodes a short polypeptide which can be specifically recognized and spliced by DL-Dithiothreitol（DTT）. In our case we add this short sequence between the recombinant protein which we want to purify and the linker protein Spytag, in order to separate the recombinant protein form the Magnetosome.
Notes
Our project is to use Magnetosome to purify recombinant protein, and we need to remove the protein from the compound after the protein we are interested combined with Magnetosome. As this short polypeptide can be spliced by DTT, we can meet this need by adding the intein between the recombinant protein and magnetosome.
Source
This sequence comes form the gyrA gene of Mycobacterium xenopi
