BBa_K1947004

BBa_K1947004 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K1947004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jie Tang
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-14 02:56:39

This part serves as a catch system expressed in AMB-1.



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1947004_sequence (Version 1)

Description

Mms13 is a protein that bound to magnetosome directly and tightly on a bilayer phospholipid membrane of the bacterial magnetic particles (BMPs). GS linker is used to construct the fusion protein to prevent the two protein from influencing each other. And there is a highly specific recognition and covalent conjugation between Spytag (a peptide with 13 amino acids) and Spycatcher (a small protein consisting of 138 residues). The Spycatcher-Mms13-linked Magnetosome can specifically and covalently conjugate to the Spytag-tagged protein in the bacterial lysate and they can be simply co-purified by a magnet.

Notes

We add GS linker to prevent two protein???s conformation from influencing each other.

Source

Magnetic bacterium Magnetospirillum magneticum AMB-1 that can produce magnet particles, also called Magnetosome, covered by a bilayer phospholipid membrane, in which Mms13 protein is anchored;Streptococcus pyogenes fibro-nectin-binding protein FbaB contains a domain with a spontaneous isopeptide bond between Lys31 and Asp117. This domain can be splitted into two fragments: the small one is named as Spytag, while the big one is Spycatcher. Spyatcher is separated from the second immunoglobulin-like collagen adhesin domain (CnaB2) which is come from the fibronectin binding protein FbaB.

Access Instance Definition
public
BBa_K1947000
Mms13
Sequence Annotation Location Component / Role(s)
BBa_K1947000
1,378
Mms13
igem#experience
None
 
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1947004/1