BBa_K1947021

BBa_K1947021 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K1947021
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jie Tang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 01:58:26

This part serves as a catch system expressed in E. coli.



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1947021_sequence (Version 1)

Description

There is a highly specific recognition and covalent conjugation between Spytag (a peptide with 13 amino acids) and Spycatcher (a small protein consisting of 138 residues). A protein of interest with a Spytag at its N- or C-terminus is expressed in E. coli and present in bacterial lysate.

Notes

We add one glycine between Spytag and TEV site to prevent two short peptides from influencing each other.

Source

Streptococcus pyogenes fibro-nectin-binding protein FbaB contains a domain with a spontaneous isopeptide bond between Lys31 and Asp117. This domain can be splitted into two fragments: the small one is named as Spytag, while the big one is Spycatcher. Spytag is separated from the second immunoglobulin-like collagen adhesin domain (CnaB2) which is come from the fibronectin binding protein FbaB.

igem#experience
None
 
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1947021/1