BBa_K1947022

BBa_K1947022 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K1947022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jie Tang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-17 10:55:52

Improve the protein purification.



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1947022_sequence (Version 1)

Description

TEV protease may bring some impure proteins. We use intein to replace the TEV site. The M. xenopi GyrA intein provides a paradigm for a minimal protein splicing element. Intein can be cleaved by DTT. DTT as reducing agent protects the free thiol groups of the protein from oxidation and so it doesn???t bring foreign substance. As a result, intein can achieve a better effect of purification than TEV protease.

Notes

GS linker is used to construct the fusion protein. Amilcp does not have a termination  codon.

Source

Protein splicing elements (termed inteins) were described as in-frame insertions in the Saccharomyces cerevisiae VMA1 gene.

igem#experience
None
 
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1947022/1