Types | DnaRegion
|
Roles | engineered_region
Composite
|
Sequences | BBa_K1947022_sequence (Version 1)
|
Description
TEV protease may bring some impure proteins. We use intein to replace the TEV site. The M. xenopi GyrA intein provides a paradigm for a minimal protein splicing element. Intein can be cleaved by DTT. DTT as reducing agent protects the free thiol groups of the protein from oxidation and so it doesn???t bring foreign substance. As a result, intein can achieve a better effect of purification than TEV protease.
Notes
GS linker is used to construct the fusion protein. Amilcp does not have a termination codon.
Source
Protein splicing elements (termed inteins) were described as in-frame insertions in the Saccharomyces cerevisiae VMA1 gene.