Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jie Tang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 02:33:09
This part serves as a catch system expressed in AMB-1.
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1947025_sequence (Version 1) |
Description
Mms13 is a protein that bound to magnetosome directly and tightly on a bilayer phospholipid membrane of the bacterial magnetic particles (BMPs). GS linker is used to construct the fusion protein to prevent the two protein from influencing each other. And there is a highly specific recognition and covalent conjugation between Spytag (a peptide with 13 amino acids) and Spycatcher (a small protein consisting of 138 residues). The Spycatcher-Mms13-linked Magnetosome can specifically and covalently conjugate to the Spytag-tagged protein in the bacterial lysate and they can be simply co-purified by a magnet.Notes
We add GS linker to prevent two protein???s conformation from influencing each other.Source
Magnetic bacterium Magnetospirillum magneticum AMB-1 that can produce magnet particles, also called Magnetosome, covered by a bilayer phospholipid membrane, in which Mms13 protein is anchored；Streptococcus pyogenes fibro-nectin-binding protein FbaB contains a domain with a spontaneous isopeptide bond between Lys31 and Asp117. This domain can be splitted into two fragments: the small one is named as Spytag, while the big one is Spycatcher. Spyatcher is separated from the second immunoglobulin-like collagen adhesin domain (CnaB2) which is come from the fibronectin binding protein FbaB.| Access | Instance | Definition |
| public public public | BBa_K1947000 BBa_K1486004 BBa_K1159200 | Mms13 BBa_K1486004 BBa_K1159200 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1947000 BBa_K1486004 BBa_K1159200 | 1,378 387,416 425,763 | Mms13 BBa_K1486004 BBa_K1159200 |